Journal: Scientific Reports

Article Title: UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway

doi: 10.1038/s41598-025-86496-9

Figure Lengend Snippet: Hinokitiol could improve renal fibrosis in UUO mice. ( A ) Experimental design. Red dots indicate the timing of UUO surgery. Mice were sacrificed 7 days after UUO surgery. Grey arrows indicate injections of Hinokitiol (25 mg/kg). ( B ) Representative micrographs showing renal expression of Masson’s trichrome staining, Fibronectin, Collagen III and P-SMAD3 in different groups. Red arrows indicate tubular dilatation. Scale bar = 50 μm. ( C ) Quantitative determination of fibrotic areas and the expression of Fibronectin, Collagen III and P-SMAD3 in different groups. ( D ) Representative Western blot showing the expression of E-cadherin, N-cadherin, Vimentin, Snail1, α-SMA and UHRF1 in different groups. ( E ) Representative Western blot showing the expression of P-SMAD3, SMAD3, P-SMAD2 and SMAD2 in different groups. Numbers (1–3) indicate each individual animal in a given group. ( n = 3 per group, data are expressed as mean ± SD. *P <0.05, * *P <0.01, ** *P <0.001)

Article Snippet: Mice were randomly divided into three groups ( n = 6): Sham group, UUO group, and UUO + Hinokitiol group, with Hinokitiol (HY-B2230, MedChemExpress; 25 mg/kg) injected intraperitoneally one day before and three days after ligation, and an equal amount of solvent injected in the Sham and UUO groups.

Techniques: Expressing, Staining, Western Blot

Journal: PLoS ONE

Article Title: Inhibition of RIP1-RIP3-mediated necroptosis attenuates renal fibrosis via Wnt3α/β-catenin/GSK-3β signaling in unilateral ureteral obstruction

doi: 10.1371/journal.pone.0274116

Figure Lengend Snippet: Representative photomicrographs of HE staining (A), transmission electron microscopy (B), and immunoblotting analysis of RIP1-RIP3-MLKL protein in vivo (C) and in vitro (D). UUO caused necrosis within the tubular epithelium (circle) and abscission of microvilli in epithelial cells (arrows), whereas these changes were attenuated by RIP inhibitor or ICG-001. *** P < 0.01 vs. sham or control; # P < 0.05 vs. UUO or H 2 O 2 .

Article Snippet: Following 1 week acclimatization, weight-matched rats were randomized into five groups and treated daily for 7 days: 1) sham group, sham operated rats without treatment; 2) UUO group, UUO rats without treatment; 3) UUO+Necrostatin-1 (UUO+Nec-1), UUO rats received Nec-1 (2mg/kg oral gavage, HY-15760, MedChemExpress, USA [ ]); 4) UUO+ GSK872 group, UUO rats received GSK872 (1mg/kg intraperitoneal, HY-101872, MedChemExpress, USA [ ]); 5) UUO+ICG-001 group, UUO rats received ICG-001 (5mg/kg intraperitoneal, HY-14428, MedChemExpress, USA [ ].

Techniques: Staining, Transmission Assay, Electron Microscopy, Western Blot, In Vivo, In Vitro